Easy mnemonic to remember acid fast organisms is explained in this video. Acid fast (Ziehl Neelsen) staining organisms are mycobacteria, nocardia, cryptosporidium oocyst & isospora oocyst. These organisms are frequently tested in various competitive exams like USMLE and other examinations. Subscribe to our channel:https://www.youtube.com/channel/UCtU1y_tzgmhzV5qQj9Blb2A?sub_confirmation=1 Links for related playlists: 1. Mnemonics:https://www.youtube.com/playlist?list=PLbilivK1P_9LxDii1hRMDej0BspYPu6ai 2. Microbiology: https://www.youtube.com/playlist?list=PLbilivK1P_9JFZNyuTCCJfuNsxR_xb3_e 3. High Yield: https://www.youtube.com/playlist?list=PLbilivK1P_9KrZLVym2_xeIeGjlpQ4SJP 4: All Playlists:https://www.youtube.com/view_all_playlists Follow us on facebook: https://www.facebook.com/extensivemedicine/ https://www.facebook.com/ExtensiveMedicalMCQs/ Follow us on Twitter: https://twitter.com/EM_Medicine For suggestions and queries: [email protected]
Views: 680 Extensive Medicine
Tuberculosis is an infectious disease, which is a huge problem all over the world and roughly one third of the world population is infected with it. It is caused by Mycobacterium tuberculosis - acid-fast bacteria. Here we discuss histological staining to visualize acid-fast bacteria in tissues - Ziehl-Neelsen staining.
Views: 3868 BioVitrum
Hi my dear viewers, I am undergraduate medical laboratory science (BSc. SP) student in university of Peradeniya in Srilanka According to one of my friend idea I decided to start this MEDI LAB ACADEMY you tube channel.so my dear viewers mainly I did that to share my knowledge gained from consultant doctors, qualified lectures ,reference books, and during my clinical training in hospital. Actually I covered some Hematology, Bio chemistry, Histology, Parasitology and other relevant subjects related to MLT, MLS ,nursing, and even medical students. And also I created this video very simple manner .use very simple English language, clear pictures, and I hundred percent assured about the content of the my videos. In future I hope to add more and more videos helpful to your academic purposes.so I would like to invite to you all watch my videos via you tube and update your , refresh your knowledge and gather knowledge. After watching if you get some thing from my videos or if you are interesting about my videos please don’t forget to subscribe me. As well as give your comments also.it will be helpful to expand my channel, encourage me. So my dear viewers, if you have ability help me I would like to kindly accept it ,even a 1 dollar given by you will important to my higher education and build up my future .this video is small description about acid fast staining method.i included some facts you don 't know about acid fast staining method. i would like to invite you to watch this video and enjoy. key words for this video......... important facts in zeihl neelsen staining. acid fast bacilli acid fast bacteria acid fast staining in english acid fast staining in micro biology mycobacterium tuberculosis mycobacterium lepare ziehl neelsen staining. mlt. mls. special staining. THANK YOU FOR ALL
Views: 79 Medi Lab Academy
By Grp F5 this video describes the details of ziehl-nelsen and kinyoun method.
Views: 182 GNIPST B.PHARM 2017-2021 SECTION-B
The Ziehl–Neelsen stain, also known as the acid-fast stain. It is a special bacteriological stain used to identify acid-fast organisms, mainly Mycobacteria. The procedure is adopted by RNTCP for primary level diagnosis of tuberculosis in India.
Views: 2685 Tuberculosis Diagnosis
Red solid bacilli, some are beaded forms, occuring single also in masses i.e. globi 6+ because more than 1,000 bacilli , clumps and globi in every field
Views: 1190 Microhub Plus
This four and half minute video shows how to prepare an Acid-Fast Stain using the Ziehl-Nielsen Method. This resource was developed by Charles Adjei Osei of the Kwame Nkrumah University of Science and Technology and Cary Engleberg of the University of Michigan. It is part of a larger learning module about laboratory methods for clinical microbiology. The full learning module, editable animation, and video transcript are available at http://open.umich.edu/education/med/oernetwork/med/microbiology/clinical-microbio-lab/2009. Copyright 2009-2010, Kwame Nkrumah University of Science and Technology and Cary Engleberg. This is licensed under a Creative Commons Attribution Noncommerical 3.0 License http://creativecommons.org/licenses/by-nc/3.0/. Video transcribed by Andrea Matsumoto (University of Michigan). Help us caption & translate this video! http://amara.org/v/BWPk/
Views: 6963 openmichigan
Nocardia is also Acid Fast bacteria and thus it becomes positive i.e pink or red in Acid fast staining/Ziehl-Neelsen staining. Similarly it also displays a reddish-yellow fluorescence as in Mycobacterium. Nocardia is Auramine–rhodamine stain positive as shown in video. #Nocadiafluorescence #AuraminrhodamineStain #NocardiaPositive
Views: 37 Microhub Plus
Please watch: "Chicken pox" https://www.youtube.com/watch?v=rvWo141B-ZI -~-~~-~~~-~~-~- Stool from HIV patient tested aseptically ....
Views: 336 Microhub Plus
In this video we show you how to prepare a sputum smear slide and stain it using the Ziehl Neelsen technique This video was made for the Automatic Digital Microscope project. if you have any questions feel free to send a message or ask in the comment section.
Views: 15299 Mirko Párraga
The IDEXX Filta-Max xpress® system is a rapid, efficient technology for the capture and recovery of Cryptosporidium spp and Giardia spp. This video is part 4 in a 6-part series and features immunomagnetic separation: the use of antibody-coated paramagnetic beads to efficiently separate Cryptosporidium oocysts and Giardia oocysts from the water concentrate. To learn more, please visit www.idexx.com/water.
Views: 1046 IDEXX Water Microbiology
Handwritten video lecture on Tuberculosis for USMLE Step 1 and USMLE Step 2. Reviewing Pathogenesis, Signs and Symptoms, Investigations, and Treatment. BACTERIOLOGY Tuberculosis (TB) is caused by mycobacterium tuberculosis with its main virulence factor is due to its mycolic acid coat that increases immune resistance but the bacteria grows much slower. Ziehl-Neelson staining aka Acid Fast stain can stain tuberculosis, but also stains nocardia, legionella, isospora, and cryptosporidium. Tuberculosis (TB) bacteria is also aerobic. PATHOGENESIS EXPOSURE – Tuberculosis (TB) bacteria must first go into the lower respiratory tract. Generally prefers upper part of the middle and lower lobe leading to primary tuberculosis infection. MACROPHAGE – undergoes phagocytosis by macrophage and the tuberculosis will be inside of phagosome. Normally the lysosome needs to fuse to form phagolysosome. This can be inhibited by tuberculosis bacteria so over time the mycobacterium grows within the phagosome until rupture. Eventually the macrophage will rupture which will then be taken up by macrophages again. GRANULOMA – Granuloma formed when the number of macrophages with mycobacterium increases. With Cell Mediated Immunity (CMI) activation by T cells release lymphokines that activate macrophages which kills the Tuberculosis (TB) and creates casseous necrosis in the central area. Many of the tuberculosis (TB) bacteria are not killed and remain dormant. SYMPTOMS Range from asymptomatic to fever, pleuritic and chest pain. Erythema nodosum, phlyctenuclar conjunctivitis may also be seen. Early there is non-purulent cough that may become plurulent with blood. May also have pleuritic chest pain with dyspnea and respiratory distress. GHON FOCUS – ghon focus is an actual lesion that is found in the peripheral part of the lung. After some time may travel to hilar lymph node forming the ghon complex. DORMANT – There is granuloma formation which has live tuberculosis (TB) bacteria. This may reactivate and is known as reactivation TB, Post primary TB, or Secondary TB. Reactivation occurs when the patient later becomes immunocompromised. Location is in upper/posterior lobe because of increased oxygen found. PROGRESS – Tuberculosis (TB) grows and creates cavity that enlarges. Goes to airways and patient is infectious. If goes to pleura may cause pleural effusion. Hilar lymphadenopathy that may press on trachea. May go to blood vessels and create disseminated tuberculosis. COURSE – galloping consumption is when it becomes very severe. Consumption is when it takes a chronic course and finally spontaneous remission. DIAGNOSIS CHEST X-RAY – May see cavitation, Ghon complex, and calcified nodule Acid Fast Bacilli (AFB) Microscopy – Done with sputum sample or bronchioalveolar lavage (BAL). Gastric can give high false positive with Ziehl Neelsen Stain CULTURE – With Lowenstein Jensen, but takes 4-8 weeks to grow. MOLECULAR TESTS- Include nucleic acid amplification, PCR, MTB and RIF. TUBERCULIN SKIN TEST (PPD) – May detect latent stage, however, many false negatives and false positive. TREATMENT INITIAL PHASE (2 MONTHS) – attempting the kill as much bacteria as possible CONTINUATION PHASE (2-6 MONTHS) – kille persistent and resistant bacteria. Isoniazid and Rifampicin is used for 6 months. Pyrazinamide and Ethambutol is used only in first 2 months of treatment. Must monitor sputum with culture and by 3 months must be culture negative. If persistent than may be bacterial resistance or there is decreased comlaince. Also monitor Drug Toxicity which is unique for each drug. Monitor liver function test, uric acid levels, B6 levels, and optic neuritis. Direct Observation Treatment (DOT) – helped with compliance with nurse observing patient take the drug. If there is resistance can add fluoroquinolone, streptomycin, amikacin, kanamycin, capromycin. If resistant to isoniazid and rifampicin then increase treatment to 9 months. Do this also if there is cavitary lesion. Multdrug resistant Tuberculosis (TB) (MDR) you should add Fluoroquinolone and other second line drug. XDR is if there is resistance to first and second line drug.
Views: 39658 the study spot
Auramine phenol staining performed and observed under Fluorescence microscope- sputum specimen- suspected case Pulmonary tuberculosis observed at 100x; 40x and 10x objective similarly...
Views: 1904 Microhub Plus
تلوين زيل نلسن / للعصيات الصامدة للحامض / مثال مرض الدرن او السل/ عادل عبداللله
Views: 586 Labzool
When it comes to tracking infectious diseases and outbreaks, determining who is infected with a particular pathogen is the first step in solving the puzzle and stopping the spread of the disease. In this session of Public Health Grand Rounds, you will hear how culture-independent diagnostic tests are changing the landscape of diagnosing infectious disease. Comments on this video are allowed in accordance with our comment policy: http://www.cdc.gov/SocialMedia/Tools/CommentPolicy.html This video can also be viewed at https://www.cdc.gov/video/phgr/2016/GR_10-18-2016.mp4
Views: 3352 Centers for Disease Control and Prevention (CDC)
You'll Never Guess Who Has a Parasite Infection? - Podcast #86 Get Show Updates Here: http://www.beyondwellnessradio.com/newsletter You-tube Podcast Subscribe: http://www.youtube.com/subscription_center?add_user=justinhealth Show Transcription: http://justinhealth.com/youll-never-guess-parasite-infection-podcast-86/ In this podcast, Dr. Justin Marchegiani and Evan Brand reveal to us today about a health issue that Evan is experiencing currently with regard to the parasites discovered in his stool tests. Listen as they share with us the things that can be done to get rid of these parasites via conventional medicine approach versus the functional medicine methods. Discover what the common parasites are and what the conventional symptoms are that people typically experience when they have an infection. Learn the various ways and supplementation to help treat parasite infections as well as the laboratory tests you can take to confirm such infections. In this episode, topics include: 00:44 Evan’s parasite story and symptoms 5:03 Lab testing 11:28 Giardia 15:33 Cryptosporidium 22:17 Supplementation Subscribe on I-Tunes: http://www.beyondwellnessradio.com/itunes Review us at: http://www.beyondwellnessradio.com/itunes Visit us at: http://www.beyondwellnessradio.com Have a question: http://www.beyondwellnessradio.com/question -~-~~-~~~-~~-~- Please watch: "GI Issues — Malabsorption, Infection & Inflammation in the Eye and Joint | Dr. J Live Q & A" https://www.youtube.com/watch?v=dGf7F1Xi6po -~-~~-~~~-~~-~-
Views: 1431 Just In Health
John from http://www.growingyourgreens.com/ answers the question: Should I plant in the soil in my backyard or buy compost (bring in soil) to plant my organic garden? In this episode John explains what he would do (and is doing) to plant a lush, beautiful edible food vegetable garden. After watching this episode you will learn if its better to rebuild your soil or purchase soil to grow a garden in. Finally, you will learn //99about the key ingredients that are vital for healthy soil and how you can easily add them to your existing soil so you can grow some of the best vegetables and fruits on earth. Learn more about the John and Bob's Products at https://www.johnandbobs.com/purchase/... be sure to use discount code GrowGreen for 10% off your order.
Views: 89671 Learn Organic Gardening at GrowingYourGreens
In this video I go over the 6 tests that are essentCheck out my free training videos at https://www.motivated2heal.com/free-training/ Join My Facebook Group https://www.facebook.com/groups/motivated2healnaturally/ Need Help? Book a Free Health Assessment https://www.motivated2heal.com/free-20-minute-health-assessment Looking for a Practitioner? Check Out My Coaching Packages https://www.motivated2heal.com/work-with-me/
Views: 6155 Motivated 2 Heal
http://www.curamedicine.com.au Metametrix use a cutting-edge stool microbiology test for intestinal bacteria, parasites and yeast. This test uses DNA technology in which laboratory equipment detects the genetic material of individual organisms. There are a number of key advantages of this method over the old testing method of microbiological culture, most importantly the fact that it is more sensitive and avoids missing parasites in transitional life cycles. Some intestinal organisms are aerobes, which have an oxygen based metabolism, while others are anaerobes, which die when exposed to oxygen. This created problems during transport to the lab as conditions can not be ideal for all organisms, causing levels to change. This creates issues with accurate reporting in stool tests. This stool test by metametrix kills organisms in formalin upon collection and then analyses genetic material in the labatory. This means it is independent of transport and lab conditions. The DNA analysis of the stool is highly sensitive and is able to detect single genomes of organisms, whereas culture has limited sensitivity. Due to the above issues DNA analysis is able to accurately quantitate levels of organisms, whereas culture methods can not. The DNA method also has greatly increased sensitivity for detecting parasites. Using older techniques parasites are only detectable during certain stages of their life cycle and often missed. http://www.curamedicine.com.au
Who Uses a UV Water Filter System and How a UV System Work? See All Viqua UV Water Filter & Purification Systems: https://www.freshwatersystems.com/c-1339-viqua-uv-systems.aspx It looks good. It tastes OK, but is your drinking water safe for everyone to drink? Dangerous levels of harmful bacteria and viruses in drinking water cannot always be detected by sight, smell or taste. If your home’s water comes from a private source, such as a well, you are solely responsible for its disinfection and treatment. And a UV water filter system is a highly effective, chemical-free water purification method. If your water comes from a municipal or city source, the treatment plant follows rules and regulations for disinfection. Before entering your home however, your drinking water passes through miles of an aging water pipe system. Breaks in the system such as damaged pipes and broken water mains can allow dangerous contaminant into your drinking water such as bacteria and viruses - a common cause of boil water advisories. Among the ordinary events that can cause contaminant to leach into wells and surface water sources are heavy rainfall, melting snow, agricultural run-off and industrial pollution. Before it flows from your faucet, how can you make sure your water is safe to drink? Disinfect and purify your drinking water with eco-friendly UV or Ultraviolet light. What is a UV Water Purification System? Before water enters the UV chamber for purification, it needs to be clarified. A sediment pre-filter, such as a 5 micron filter, ensures the water is clear enough for effective UV light disinfection. Next, an activated carbon filter improves the water’s taste. Water then enters a stainless Steel chamber which houses the purifying UV light inside a transparent quartz sleeve. Many systems feature a 365 day counter to notify you when it is time to replace the UV lamp. How does Ultraviolet Water Treatment System purify and Disinfect Drinking Water? The germicidal energy of ultraviolet light destroys illness-causing microorganisms by attacking the DNA at their genetic core. This eliminates the organisms’ ability to reproduce, rendering them harmless. UV water purification systems eliminates 99.99% of the harm viruses and bacteria like E.coli can cause. The same is true of harmful protozoa such as Giardia and Cryptosporidium which are chlorine-resistant and a difficult challenge for many water systems. With a quality Viqua (Sterlight or UVMax) UV water filter system you can be sure your water has been disinfected, purified, and is safe to drink whether your water is from a well or municipal water supply. Learn more by visiting: http://www.freshwatersystems.com or calling 877.335.3339. Product References: UV Water Filter System for both City and Well Water: http://www.freshwatersystems.com/c-157-uv-systems.aspx Sterilight UV Water Purification System: http://www.freshwatersystems.com/c-158-sterilight.aspx UVMax Systems: http://www.freshwatersystems.com/c-632-trojan-uvmax.aspx UV Well Water System: https://www.freshwatersystems.com/t-Well-Water-Purification-Systems-and-Treatment.aspx
Views: 130710 Fresh Water Systems
This is an audio version of the Wikipedia Article: Cryptosporidiosis 00:01:04 1 Signs and symptoms 00:02:33 1.1 Intestinal cryptosporidiosis 00:03:34 1.2 Respiratory cryptosporidiosis 00:04:09 2 Cause 00:06:22 2.1 Transmission 00:08:25 2.2 Life cycle 00:10:13 3 Pathogenesis 00:11:39 4 Diagnosis 00:13:21 5 Prevention 00:17:20 6 Treatment 00:17:49 6.1 Immunocompetent 00:18:40 6.2 Immunocompromised 00:20:52 7 Epidemiology 00:21:39 8 History 00:21:55 9 Research 00:23:33 10 Other animals 00:24:05 11 Notable cases 00:24:14 11.1 Before 2000 00:25:56 11.2 2001–2009 00:30:15 11.3 2010 and later 00:32:19 12 See also Listening is a more natural way of learning, when compared to reading. Written language only began at around 3200 BC, but spoken language has existed long ago. Learning by listening is a great way to: - increases imagination and understanding - improves your listening skills - improves your own spoken accent - learn while on the move - reduce eye strain Now learn the vast amount of general knowledge available on Wikipedia through audio (audio article). You could even learn subconsciously by playing the audio while you are sleeping! If you are planning to listen a lot, you could try using a bone conduction headphone, or a standard speaker instead of an earphone. You can find other Wikipedia audio articles too at: https://www.youtube.com/channel/UCuKfABj2eGyjH3ntPxp4YeQ You can upload your own Wikipedia articles through: https://github.com/nodef/wikipedia-tts "The only true wisdom is in knowing you know nothing." - Socrates SUMMARY ======= Cryptosporidiosis, also known as crypto, is a parasitic disease caused by Cryptosporidium, a genus of protozoan parasites in the phylum Apicomplexa. It affects the distal small intestine and can affect the respiratory tract in both immunocompetent (i.e., individuals with a normal functioning immune system) and immunocompromised (e.g., persons with HIV/AIDS or autoimmune disorders) individuals, resulting in watery diarrhea with or without an unexplained cough. In immunosuppressed individuals, the symptoms are particularly severe and can be fatal. It is primarily spread through the fecal-oral route, often through contaminated water; recent evidence suggests that it can also be transmitted via fomites in respiratory secretions.Cryptosporidium is commonly isolated in HIV-positive patients presenting with diarrhea. Despite not being identified until 1976, it is one of the most common waterborne diseases and is found worldwide. The infection beings when a human consumes food or water containing cysts of the Cryptosporidium organism.
Views: 21 wikipedia tts